Dl-Dithiothreitol / DTT Briefing
English Name:DTT;DL- Dithiothreitol;1,4-Dithio-DL-threitol;Threo-1,4-dimercapto-2,3-butandiol;Threo-2,3-dihydroxy-1,4-dithiobutane;1,4-Dithiothreitol
Other names: 1,4-dithiothreitol; Dl-1,4-dithiothreitol; 1,4-dimercaptothreitol; 1,4-dimercapto-dl-threitol; Dimercaptobutanediol; 1,4-dithio-dl-butanol
CAS No.: 3483-12-3
C4H10O2S2=154.25
Level: br
Content: ≥ 99.0%
Melting point: 39 ~ 43 ºC
Moisture: ≤ 0.5%
Properties: white acicular crystal or powder. Boiling point 125 ~ 130 ºC (0.27kpa). Sublimation at 37 ºC, 0.67pa. Slightly hygroscopic, easily soluble in water, ethanol, acetone, ethyl acetate and chloroform. Slow oxidation in air
Purpose: biochemical research. Used as protein Ryukyu protective agent. It is used for cleavage and sequence analysis of protein disulfide bonds. It is also a common reducing agent with antioxidant effect. Compared with mercaptoethanol, DTT has similar effects, but the pungent smell of DTT is much smaller and the toxicity is much lower than mercaptoethanol. When the concentration of DTT was 7 times lower than that of mercaptoethanol, the effect was similar
Storage: 2 ~ 8 ºC
DL-Dithiothreitol / DTT CAS 3483-12-3 Usage:
1.molecular biology
2.Quantitative reduction of disulfides; DTT can effectively reduce protein disulfide bonds in the sample buffer before SDS-PAGE; DTT can also be used to reduce the disulfide bonds of the cross-linking agent N,N′-bis(acryloyl)cystamine Bridge to decompose polyacrylamide gel matrix; in protein sequence analysis, to remove disulfide linkages; to investigate increased and dynamic reduction reactions; to prevent the extraction of cholecystokinin from brain and duodenal tissue When the oxidation reaction occurs
Packing
· 1kg/aluminium foil bag, with two plastic bags inside.
· 25kg/fiber drum, with two plastic bags inside.
Size:ID 42cm*H52cm,0.08m3/drum;
Net Weight:25kgs Gross Weight:28kgs.
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