Tris Briefing
Tris (CAS#77-86-1), trimethylaminomethane, also known as aminotriol, ammonium bradykinin. The product is white crystals, easily soluble in water, without irritating odor. Tris is generally known as the second generation of Good’s buffer, with better solubility and stability compared to the traditional Good’s buffer.Tris is a very important biological buffer and together with Tris HCl is a commonly used buffer salt pair. tris provides a stable, weakly basic environment (7.0-9.1) that is sensitive to temperature and concentration.
Tris bases have an aqueous pH of about 10.5, and buffers of this pH are generally obtained by adding hydrochloric to adjust the pH to the desired value. EDTA is often added to Tris hydrochloric buffer to make a “TE buffer”, which is used for DNA stabilization and storage. If the acid solution used to adjust pH is replaced with acetic acid, a “TAE buffer (Tris/Acetate/EDTA) is obtained, while replacing it with boric acid gives a “TBE buffer” (Tris/Borate/EDTA) which provides a stable pH buffer environment for the reagents.
The purity of our products can reach 99% stably, and we have great shipments in China, the United States and Europe,quality and price are absolutely guaranteed and advantageous.
Application:the product can be used for the preparation of buffer solution in biochemistry and molecular biology experiment, pharmaceutical intermediate, surfactant and curing accelerator, water-soluble polymer containing Tris structure unit as paint dispersant, formaldehyde adsorption material in the room, etc.
COA OF TRIS :
Uses of Tris:
1. Chemical raw material: Tris is widely used as an important chemical raw material for flame retardant materials, coatings and amino resins, etc.
2. Reaction medium: As an excellent reaction medium, Tris is often used in organic synthesis reactions, which can play the role of catalyst.
3. Resin cross-linking agent: Tris(hydroxymethyl)aminomethane can also be used in resin cross-linking reaction, which can improve the hardness and heat resistance of resin.
4. Biological buffering agent: In the field of medicine, Tris is used as a biological buffering agent, which is widely used in cell culture, DNA/RNA extraction, PCR amplification, etc.
Configuration method of Tris solution:
1. Weigh the required amount of Tris powder and add it to sterile high purity water.
2. Use a magnetic stirrer to fully dissolve the Tris powder until it is completely homogeneous.
3. Calculation of concentration: Generally, the concentration of trimethylolaminomethane is 1 mol/L, and the concentration can be adjusted appropriately according to the need.
4. Measure the PH value of the solution with a PH meter, which should generally be controlled between 7.0-8.5.
5. Preparation of buffer solution: standardize the solution to the required volume, usually used as a buffer solution with a concentration of 50-200 mmol/L.
6. Sterilization: The trimethylolaminomethane solution is loaded into sterile bottles and sterilized according to a certain process.
It should be noted that during the configuration of trimethylolaminomethane, chemical safety operation procedures should be strictly observed, and personal protective equipment should be worn to avoid splashing the chemical on the skin and eyes, and to avoid inhalation and accidental ingestion of the chemical. At the same time, the label and production date of the chemical should be checked before use to avoid using expired or poor quality chemicals.
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